Callus induction and plant regeneration from cultured anthers of indica rice varieties [in India]

Anthers containing pollen at late uninucleate stage from cold pretreated panicles at 4-5 deg. C for 7 days of three varieties viz. Jaya, IR 54 and Vaigai were cultured on N6 medium supplemented with various combinations and concentrations of auxins, cytokinins and sucrose. The best callusing from cu...

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Bibliographic Details
Published inPlant Tissue Culture Vol. 3
Main Authors Sandhu J.S, Gill M.S, Gosal S.S
Format Journal Article
LanguageEnglish
Published 1993
Subjects
anther culture
cal
callo
callus
cultivo de anteras
culture d' anthere
inde
india
oryza sativa
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Summary:Anthers containing pollen at late uninucleate stage from cold pretreated panicles at 4-5 deg. C for 7 days of three varieties viz. Jaya, IR 54 and Vaigai were cultured on N6 medium supplemented with various combinations and concentrations of auxins, cytokinins and sucrose. The best callusing from cultured anthers occurred on N6 medium containing 2, 4-D (1.75 mg/l), Kn (0.5 mg/l), sucrose (3% w/v) ranging from 1.75 per cent in Jaya to 2.25 per cent in IR 54. Upon transfer to N6 medium supplemented with BAP (0.5 mg/l) and sucrose (4.5% w/v) anther-derived calli differentiated into shoots ranging from 15% in Jaya to 24% in IR 54.
Bibliography:F
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ISSN:1018-8029