Quantifying Absorption by Aquatic Particles: A Multiply Scattering Correction for Glass-Fiber Filters

Absorption spectra measured for aquatic particles concentrated onto glass-fiber filters require a correction for the increase in pathlength caused by multiple scattering in the glass-fiber filter. A multiple scattering correction was calculated from optical density spectral for 48 phytoplankton cult...

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Bibliographic Details
Main Authors Cleveland, J. S, Weidemann, A. D
Format Publication
LanguageEnglish
Published 14.03.2007
Subjects
ABSORPTION SPECTRA
ALGORITHMS
AQUATIC
ATOMIC AND MOLECULAR PHYSICS AND SPECTROSCOPY
CELLS
CORRECTIONS
CULTURE
DENSITY
ERRORS
FILTERS
GLASS FIBERS
NEUTRAL
OCEANOGRAPHY
OPTICS
PARTICLES
PATHLENGTHS
PE61163N
PHYSICAL
PHYSICAL AND DYNAMIC OCEANOGRAPHY
PHYTOPLANKTON
PIGMENTS
QUANTIFYING
REFRACTORY FIBERS
REGIONS
REPRINTS
SCATTERING
SPECTRA
WALLS
WUDN094463
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Summary:Absorption spectra measured for aquatic particles concentrated onto glass-fiber filters require a correction for the increase in pathlength caused by multiple scattering in the glass-fiber filter. A multiple scattering correction was calculated from optical density spectral for 48 phytoplankton cultures of seven species representing a variety of cell sizes, pigment groups, and call-wall types. The relationship between optical density in suspensions and on filters was not wavelength-dependent. Differences between blank filters were always spectrally neutral. Small differences between relationships for single species were inconclusive. Given the absence of wavelength-dependent effects, we report a single general quadratic relationship, OD(susp)(lambda) = 0.378 OD(filt)(lambda) + 0.523 OD(filt)(lambda)2 (r2 = 0.988), for correcting glass- fiber filter spectra. For independent samples, the average error in predicting OD(susp)(lambda) with this algorithm at any wavelength was 2%. Greatest errors were in spectral regions of low absorption. Absorption spectra for particles concentrated onto glass-fiber filters can be quantitatively corrected for multiple scattering within this limit. Applicability of the algorithm to field samples of varied composition was enhanced by using a large number of spectra and a range of cell types in algorithm development. Optical oceanography, Physical oceanography, Absorption, Scattering. Availability: Pub. in Limnology Oceanography, v38 n6 p1321-1327, 1993.
Bibliography:ADA275351