Process of inactivation of genes which codify for enzymes of the catabolysm of phenylacetate plasmides which intervene and strains transformed with the same

Procedure for the inactivation of genes that code for phenylacetate catabolism enzymes, plasmids containing them, and strains transformed with them. The procedure is preferably applied to the phacA gene of A. nidulans and to the pahA gene of P. chrysogenum, genes which code respectively for enzymes...

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Main Authors JOSE MANUEL MINGOT ASCENCAO, MIGUEL ANGEL PENALVA SOTO, FRANCISCO SALTO MALDONADO, ALEJANDRO VITALLER ALBA, JOSE MANUEL FERNANDEZ-CANON, BRUNO DIEZ CARCIA, JOSE LUIS BARREDO FUENTE, MARTA RODRIGUEZ SAIZ
Format Patent
LanguageEnglish
Published 22.10.1998
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Procedure for the inactivation of genes that code for phenylacetate catabolism enzymes, plasmids containing them, and strains transformed with them. The procedure is preferably applied to the phacA gene of A. nidulans and to the pahA gene of P. chrysogenum, genes which code respectively for enzymes competing for this substrate with penicillin biosynthesis enzymes. The non-expression of these enzymes has a beneficial effect on the synthesis of this antibiotic, increasing its yield, with a lower phenylacetate demand per unit of culture.
Bibliography:Application Number: ZA19980003216