Dextranase enzyme method for its production and DNA encording the enzyme

A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungu...

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Main Authors MARIA ELENA GONYALEZ MARTINEZ, EFRAIN RODRIGUEZ JIMENEZ, ROSSANA GARCIA FERNANDEZ, JOSE ALBERTO CREMATA ALVAREZ, DANIA MATEU CURBELO, HERNAN ROCA CAMPANA, VIVIAN MORERA CORDOVA, BIANCA MARIA GARCIA GARCIA, JULIO MARCOS DELGADO BOADA, MANUEL RAFAEL RAICES PEREZ-CASTANEDA, LUIS S HERRERA MARTINEZ, EMILIO MARGOLLEZ CLARK, CARLOS FERNANDEZ PADRON
Format Patent
LanguageEnglish
Published 05.02.1996
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.
Bibliography:Application Number: ZA19940009799