PLANT TISSUE CULTURE MEDIUM AND PREPARATION METHOD

Provided are a plant tissue culture medium, and a preparation method therefor and an application thereof. According to the present invention, a nitrogen source in calcium ammonium nitrate (5Ca(NO3)2.NH4NO3.10H2O) is used for replacing nitrogen sources provided by ammonium nitrate (NH4.NO3) and potas...

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Bibliographic Details
Main Authors YANG, Benpeng, WU, Yuanli, ZHANG, Shuzhen, CAO, Zhengying, ZAN, Limei
Format Patent
LanguageEnglish
Published 31.05.2023
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Summary:Provided are a plant tissue culture medium, and a preparation method therefor and an application thereof. According to the present invention, a nitrogen source in calcium ammonium nitrate (5Ca(NO3)2.NH4NO3.10H2O) is used for replacing nitrogen sources provided by ammonium nitrate (NH4.NO3) and potassium nitrate (NH4.NO3) in an MS culture medium, and moreover, due to the introduction of calcium ammonium nitrate, the original concentrations of macro elements and calcium ions are changed, so that proper ammonium sulfate, potassium chloride and potassium sulfate are introduced during preparation; the ratio of the macro elements to calcium elements is consistent.
Bibliography:Application Number: ZA20230002409