BREEDING METHOD USING RICE INDICA-JAPONICA INTERSUBSPECIFIC TETRAPLOID RECOMBINANT INBRED LINES

• Main Authors: Jian, MA, Jiahao, LI, Ting, LUO, Bao, LIU, Xiuyun, LIN, Lei, GONG, Ying, WU, Yue, SUN, Guo, LI, Xu, HAN
• Format: Patent
• Language: English
• Published: 25.01.2023

Disclosed in the present invention is a method for breeding using tetraploid recombinant inbred lines obtained by hybridization/doubling of rice indica and japonica subspecies. The method of the present invention comprises: hybridizing indica rice with japonica rice; doubling the hybrid into a tetraploid; inbreeding the tetraploid and selecting tetraploid recombinant inbred lines having target traits; and obtaining, by means of chromosome set ploidy reduction, a diploid having target traits, i.e., a rice breeding material having targeted traits. According to the present invention, the sterility of F1 hybrids of indica rice and japonica rice is overcome; a tetraploid is taken, by using the high tolerance of the tetraploid to the instability of a genome, as a platform that can quickly induce a large number of genomic variations; tetraploid recombinant inbred lines having diverse phenotypes and genomes subject to genomic variations such as fragment exchange between partially homologous chromosomes are quickly obtained from inbred progenies of the tetraploid; diploid restorer lines induced by means of chromosome set ploidy reduction can mostly inherit the phenotypes and resistance characteristics of the parental tetraploid of the diploid restorer lines. The present invention has important significance for rice breeding.