PESTICIDAL TOXINS AND NUCLEOTIDE SEQUENCES WHICH ENCODE THESE TOXINS

• Main Authors: FINSTAD-LEE, STACEY, LOEWER, DAVID, NARVA, KENNETH, E, STAMP, LISA, FEITELSON, JERALD, S, MULLER-COHN, JUDY, MORRILL, GEORGE, DULLUM, CHARLES, JOSEPH, SCHNEPF, H., ERNEST, SCHMEITS, JAMES, STOCKHOFF, BRIAN, A
• Format: Patent
• Language: English; French
• Published: 11.11.1999
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Disclosed and claimed are novel Bacillus thuringiensis isolates, pesticidal toxins, genes, and nucleotide probes and primers for the identification of genes encoding toxins active against pests. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The subject invention provides entirely new families of toxins which can be obtained from the supernatants of Bacillus cultures. L'invention concerne de nouveaux isolats de Bacillus thuringiensis, des toxines pesticides, des gènes, ainsi que des sondes et des amorces nucléotidiques utiles pour l'identification de gènes codant pour des toxines actives contre des ravageurs. Les amorces sont utiles dans des techniques de PCR pour produire des fragments de gène qui sont caractéristiques de gènes codant pour ces toxines. L'invention présente des familles totalement nouvelles de toxines qui peuvent être obtenues à partir de surnageants de cultures de Bacillus.