DIISOPROPYL FLUOROPHOSPHATASE AND THE UTILIZATION AND PRODUCTION THEREOF

• Main Authors: RUETERJANS, HEINZ, DIERL, STEFAN
• Format: Patent
• Language: English; French; German
• Published: 09.03.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DISPOSAL OF SOLID WASTE; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR; HUMAN NECESSITIES; HYGIENE; LAUNDERING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECLAMATION OF CONTAMINATED SOIL; RECLAMATION OF CONTAMINED SOIL SOIL; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TEXTILES; TRANSPORTING; TREATMENT OF TEXTILES OR THE LIKE; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE; TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS,FABRICS, FEATHERS, OR FIBROUS GOODS MADE FROM SUCH MATERIALS; VINEGAR; WINE

Die vorliegende Erfindung betrifft eine Diisopropylfluorophosphatase (Enzymklassifikation EC 3.1.8.2.) aus Loligo vulgaris, und die das Enzym kodierende Basensequenz. Die Erfindung betrifft des weiteren Vektoren, welche die erfindungsgemässe DNA-Sequenz enthalten, sowie mit der erfindungsgemässen Basensequenz transformierte Zellen. Darüber hinaus betrifft die Erfindung ein Verfahren zur Herstellung der Diisopropylfluorophosphatase, welches sich der erfindungsgemässen transformierten Zellen bedient. Die Erfindung betrifft auch verschiedene Verwendungsarten und Einsatzgebiete der DFPase, insbesondere die Dekontamination verseuchter Lebensräume, die Herstellung von Arzneimitteln zur Behandlung bzw. Entgiftung von Mensch und Tier, die Imprägnierung von Kleidung und den Einsatz in der Analytik. The invention relates to diisopropyl fluorophosphatase (enzyme classification EC 3.1.8.2.) from Loligo vulgaris and to the base sequence which codes the enzyme. The invention also relates to the additional vectors which contain the inventive DNA sequence and to the cells transformed with the inventive base sequence. The invention provides a method for producing the diisopropyl fluorophosphatase which uses inventive transformed cells. The invention further relates to different uses and areas of application of the DFPase, especially in the decontamination of contaminated habitats, the production of medicaments for treating or detoxifying humans and animals, waterproofing of clothing and use in analytical chemistry. L'invention concerne une di-isopropylfluorophosphatase (classification des enzymes EC 3.1.8.2) issue de Loligo vulgaris, ainsi que la séquence de bases codant l'enzyme. L'invention concerne également des vecteurs contenant la séquence d'ADN de l'invention, ainsi que des cellules transformées à l'aide de ladite séquence de bases. L'invention concerne de plus un procédé de préparation de di-isopropylfluorophosphatase, qui fait appel aux cellules transformées selon l'invention. L'invention concerne pour finir différents types d'utilisation et domaines d'application de la DFPase, notamment la décontamination d'habitats contaminés, la préparation de médicaments s'utilisant pour traiter ou désintoxiquer l'homme et l'animal, l'imprégnation de vêtements et l'utilisation dans le domaine de la chimie analytique.