METHODS FOR PSEUDOADENOVIRAL VECTOR PRODUCTION

The invention is directed to helper adenoviruses which facilitate the production of pseudoadenoviral vectors (PAV) but which cannot be packaged into viral particles. The invention is further directed to novel PAV producer cell lines expressing DNA binding and/or repressor proteins that prevent the p...

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Bibliographic Details
Main Authors ROMANCZUK, HELEN, ARMENTANO, DONNA, GREGORY, RICHARD, J, WADSWORTH, SAMUEL, C
Format Patent
LanguageEnglish
French
Published 19.08.1999
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention is directed to helper adenoviruses which facilitate the production of pseudoadenoviral vectors (PAV) but which cannot be packaged into viral particles. The invention is further directed to novel PAV producer cell lines expressing DNA binding and/or repressor proteins that prevent the packaging of the helper viruses. The invention is also directed to methods for the production of PAV in such cell lines with minimal contamination from helper viruses. L'invention concerne des adénovirus auxiliaires qui facilitent la production de vecteurs pseudo-adénoviraux (PAV) mais qui ne peuvent pas être encapsidés dans des particules virales. L'invention concerne également de nouvelles lignées cellulaires de production de PAV qui expriment les protéines liantes ou les répresseurs des ADN empêchant l'encapsidation des adénovirus auxiliaires. En outre, l'invention concerne des procédés de fabrication de PAV dans ces lignées cellulaires avec un minimum de contamination due aux virus auxiliaires.
Bibliography:Application Number: WO1999US03483