EXPRESSION CLONING IN FILAMENTOUS FUNGI

• Main Authors: VAN DEN BRINK, JOHANNES, MAARTEN, SELTEN, GERARD, CORNELIS, MARIA, VAN DEN HOMBERGH, JOHANNES, PETRUS, THEODORUS, WILHELMUS
• Format: Patent
• Language: English; French
• Published: 10.09.1999
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Methods are provided for isolation of DNA sequences encoding proteins with properties of interest by means of expression cloning in filamentous fungal host cells. The isolated DNA sequences are useful in processes for producing the proteins of interest. L'invention concerne des procédés qui permettent d'isoler des séquences d'ADN codant des protéines présentant un intérêt particulier au moyen d'un clonage d'expression dans des cellules hôtes fongiques filamenteuses. Les séquences d'ADN isolées sont utiles dans les procédés de production des protéines présentant un intérêt particulier.