DETECTION OF TARGETS WITH GREEN FLUORESCENT PROTEIN AND FLUORESCENT VARIANTS THEREOF

• Main Author: PLAIA, TODD, W
• Format: Patent
• Language: English; French
• Published: 20.08.1998
• Edition: 6
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FISHING; FORESTRY; HETEROCYCLIC COMPOUNDS; HUMAN NECESSITIES; HUNTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PHYSICS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

Green fluorescent protein or fluorescent variants thereof are combined with ligands for use as labeled markers in detection of targets. Suitable ligands include nucleic acid probes, antibodies, hapten conjugates, biotin, avidin and streptavidin. Techniques such as fluorescent microscopy are used to visualize the labeled marker. On combine une protéine fluorescente verte ou ses variantes fluorescentes avec des ligands afin de les utiliser en tant que marqueurs fluorescents dans la détection de cibles. Les ligands appropriés comprennent des sondes d'acide nucléique, des anticorps, des conjugués d'haptènes, biotine, avidine et streptavidine. On met en application des techniques, telles que la microscopie fluorescente, afin visualiser le marqueur.