PRODUCTION OF POLY- beta -HYDROXYBUTYRATE IN PROKARYOTIC HOST CELLS

The present invention provides methods for the production of poly- beta -hydroxybutyrate, comprising the steps of (a) introducing into a prokaryotic host cell capable of metabolizing sucrose a vector construct which directs the expression of a sequence which encodes a poly- beta -hydroxybutyrate bio...

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Bibliographic Details
Main Authors DENNIS, DOUGLAS, E, SLATER, STEVEN, C
Format Patent
LanguageEnglish
French
Published 29.09.1994
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides methods for the production of poly- beta -hydroxybutyrate, comprising the steps of (a) introducing into a prokaryotic host cell capable of metabolizing sucrose a vector construct which directs the expression of a sequence which encodes a poly- beta -hydroxybutyrate biosynthetic pathway, (b) culturing the host cell in medium containing sucrose, and (c) isolating poly- beta -hydroxybutyrate from the cultured host cell. Procédé d'obtention de poly-beta-hydroxybutirate consistant à: (a) introduire dans une cellule-hôte procariotique capable de métaboliser la sucrose dans un vecteur de recombinaison, commandant l'expression d'une séquence codant pour une chaîne poly-beta-hydroxybutyrate biosynthétisée, (b) mettre en culture la cellule-hôte, dans un milieu contenant de la sucrose, (c) isoler le poly-beta-hydroxybutirate de la cellule hôte cultivée.
Bibliography:Application Number: WO1994US03252