ANTI-HIRUDIN POLYCLONAL ANTIBODIES AND THEIR USE FOR THE IDENTIFICATION, IMMUNOPURIFICATION AND QUANTITATIVE DETERMINATION OF HIRUDIN

• Main Authors: GERNA, MARCO, GIORGETTI, CARLA, LANSEN, JACQUELINE, MOLINARI, ANTONIO, RONCUCCI, ROMEO
• Format: Patent
• Language: English
• Published: 07.01.1993
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a method of producing an immunogen, which method comprises polymerising a hirudin and/or a hirudin-like protein. Typically this is achieved by incubation with glutaraldehyde. The immunogen produced can be used to produce polyclonal or monoclonal antibody capable of binding to hirudin or a hirudin-like protein. Procédé de production d'un immunogène, consistant à polymériser une hirudine et/ou une protéine semblable à l'hirudine. Habituellement, ceci s'effectue par incubation avec du glutaraldéhyde. L'immunogène ainsi obtenu peut être utilisé pour produire un anticorps polyclonal ou monoclonal pouvant se lier à l'hirudine ou à une protéine semblable à l'hirudine.