FSH-RELEASING PEPTIDE

A 28,000-dalton protein with FSH-releasing activity is isolated from porcine follicular fluid in a multi-step procedure including gel filtration, ion exchange chromatography and several reverse-phase high-performance liquid chromatography steps. The 28 kD protein promotes secretion of FSH, but not o...

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Main Authors YING, SHAO-YAO, LING, NICHOLAS, CHAI-KWAN, RIVIER, JEAN, EDOUARD, FREDERIC, ESCH, FREDERICK, STEPHEN, GUILLEMIN, ROGER, CHARLES, LOUIS, VALE, WYLIE, WALKER, JR, SPIESS, JOACHIM
Format Patent
LanguageEnglish
Published 08.10.1987
Edition4
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Summary:A 28,000-dalton protein with FSH-releasing activity is isolated from porcine follicular fluid in a multi-step procedure including gel filtration, ion exchange chromatography and several reverse-phase high-performance liquid chromatography steps. The 28 kD protein promotes secretion of FSH, but not of LH, in a rat anterior pituitary monolayer culture system, exhibiting an EC50 of 0.5 ng/ml. Une protéine de 28.000 daltons présentant un effet stimulateur de l'hormone foliculo-stimulante est isolée à partir du fluide foliculaire porcin au cours d'un procédé à plusieurs étapes comprenant une filtration sur gel, une chromatographie à échange d'ions et plusieurs étapes de chromatographie en phase liquide inversée de haute performance. La protéine de 28 kD stimule la sécrétion de FSH, mais non pas celle de l'hormone lutéinisante LH, dans un système de culture monocouche du lobe antérieur d'une glande pituitaire de souris, présentant un EC50 de 0,5 ng/ml.
Bibliography:Application Number: WO1987US00790