CYSTEINE-DEPLETED MUTEINS OF BIOLOGICALLY ACTIVE HUMAN TUMOR NECROSIS FACTOR PROTEINS

• Main Authors: MARK, DAVID, F, LU, SHI-DA, YU, WANG, ALICE, M, LIN, LEO, S
• Format: Patent
• Language: English
• Published: 14.08.1986
• Edition: 4
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Synthetic muteins of biologically active human tumor necrosis factor (hTNF) wherein at least one of the two cysteine residues is deleted or replaced by another amino acid to eliminate sites for intermolecular crosslinking. The invention also relates to synthetic structural genes having DNA sequences that have been specifically designed to encode the above-described synthetic hTNF muteins. Mutéines synthétiques du facteur nécrotique tumoral humain biologiquement actif (hTNF) où au moins l'un des deux résidus de cystéine est effacé ou remplacé par un autre acide aminé pour éliminer les sites de réticulation intermoléculaire. L'invention se rapporte également aux gènes synthétiques structuraux possédant des séquences d'ADN conçues spécifiquement pour le codage des mutéines synthétiques hTNF ci-décrites.