POLYSACCHARIDE PS-A ISOLATED FROM THE PLANT OF GENUS EPIMEDIUM VIOLACEUM MORR. ET DECNE., PROCESS FOR ITS PREPARATION, AND INFECTION-PREVENTING AGENT AND IMMUNOSTIMULATING AGENT CONTAINING THE PLOYSACCHARIDE AS EFFECTIVE INGREDIENT

• Main Authors: YOSHIOKA KIMITOMO, MITSUHASHI SUSUMU, WASHIZAWA ICHIRO, YASUI SOSUKE, TAKASE MUNEAKI
• Format: Patent
• Language: English; Japanese
• Published: 11.11.1982
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

Polysaccharide PS-A isolated from the plant of genus Epimedium violaceum Morr. et decne. having the following properties, process for its preparation, and an infection-preventing agent and an immunostimulating agent containing same as an effective ingredient: 1. Elemental analysis: C=40.92, H=6.17, ash= trace; 2. Molecular Weight: 75,000 + 25,000 (mean molecular weight); 3. Decomposition point: 205C; 4. pH: 7.0 (100 mg of PS-A in 50 ml of water); 5. Specific rotary power: [ alpha ] = -23.6 (in H2O, c = 0.527); 6. IR absorption spectrum: nu KBr max (cm<-1>): 3400, 2900, 1620, 1400, 1230, 1060; 7. UV absorption spectrum: no maximum absorptions in 240 to 400 nm; 8. Appearance: white to slightly brown amorphous powder; 9. Solubility: soluble in water, insoluble in methanol, ethanol, acetone, ethyl, acetate, diethyl ether, hexane, and chloroform; 10. Color reaction: positive for the following reactions: (a) anthrone test, (b) Molisch reaction, (c) skatole reaction, (d) Bial reaction; negative for the following reactions: (a) ninhydrin reaction, (b) 2,4-DNP reaction, (c) Selivanoff reaction, (d) naphthoresorcin reaction, (e) carbazole reaction; 11. Constitutive sugar: arabinose and galactose; 12. Uniformity, uniform in ultracentrifugation, electrophoresis, and gel filtration. Polysaccharide PS-A isole a partir de la plante appartenant au genre Epidemium violaceum Morr. et Decne, possedant les proprietes suivantes, procede de preparation de ce polysaccharide, et agent de prevention de l'infection et immunostimulant contenant ce polysaccharide a titre d'ingredient actif: 1. Analyse elementaire: C = 40,92, H = 6,17, cendres = traces; poids moleculaire: 75.000+ 25.000 (poids moleculaire moyen); temperature de decomposition: 205 C; pH: 7,0 (100mg de PS-A dans 50 ml d'eau); pouvoir rotatoire specifique: (((Alpha))19 = -23,6 (dans H2O, C = 0, 527); spectre d'absorption infrarouge: (Alpha) max (cm-1): 3400, 2900, 1620, 1400, 1230, 1060; spectre d'absortion dans l'ultraviolet: pas de maximums d'absorption dans la bande de 240 a 400 nm; aspect: poudre amorphe de couleur blanche a legerement brune; solubilite: soluble dans l'eau, insoluble dans le methanol, l'ethanol, l'acetone, l'acetate d'ethyle, le diethyle ether, l'hexane et le chloroforme; reactions colorees: positives dans les reactions suivantes: a) test a l'anthrone, b) reaction de Molisch, c) reaction au scatole, d) reaction de Bial, et negatives dans les reactions suivantes: a) reaction a la ninhydrine, b) reaction au 2,4-DNP, c) reaction de Selivanoff, d) reaction a la naphtoresorcine, e) reaction au carbazole; sucres constitutifs: arabinose et galactose; uniformite: uniforme a l'ultracentrifugation, l'electrophorese et la filtration sur gel.