SEMI-SOLID PHASE EXTRACTION OF NUCLEIC ACIDS USING A QUATERNARY SALT AND CATION MIXTURE IN ALKALINE CONDITIONS

• Main Authors: HAESENDONCKX, Max, VANKERCKHOVEN, Eef, IVENS, Tania, BARAULT, Ludovic, GILLIAERT, Kristof
• Format: Patent
• Language: English; French
• Published: 31.10.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The present invention relates to improved methods for purifying nucleic acids comprising quaternary ammonium salts and free cations at alkaline conditions, requiring less, less costly and less noxious materials than extraction methods based on highly chaotropic salts. The method is broadly applicable but 5 especially useful in micro-fluidic cartridges. La présente invention concerne des procédés améliorés de purification d'acides nucléiques comprenant des sels d'ammonium quaternaire et des cations libres dans des conditions alcalines, nécessitant des matériaux en moindre quantité, moins coûteux et moins nocifs que les procédés d'extraction basés sur des sels hautement chaotropiques. Le procédé est largement applicable mais particulièrement utile dans des cartouches microfluidiques.