METHOD FOR CONSTRUCTING AND CULTURING HAIR FOLLICLE ORGANOIDS, AND HAIR DRUG SCREENING METHOD USING SAME, AND HAIR TRANSPLANT MATERIAL

• Main Authors: KANG, Kyung-Sun, KIM, Minji
• Format: Patent
• Language: English; French; Korean
• Published: 19.09.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to a technology for constructing and culturing hair follicle organoids for hair drug screening methods and production of hair transplant materials. More specifically, in the present invention, hair follicles were isolated from existing skin organoids derived from human induced pluripotent stem cells and cultured on collagen- and Matrigel-coated culture inserts to observe hair follicle growth and degeneration. The transplantation of the cultured hair follicles into the skin resulted in the growth of new hair, confirming a potential use of the hair follicle organoids as hair transplant materials. A hair loss-mimic model was generated on the basis of observing reductions in hair follicle length, damage to hair papilla cell aggregates, decreased expression of WNT3A or β-catenin, and increased expression of DKK-1 or TGF-β2 according to treatment of the hair follicle organoids with alopecia-inducing factors. Through the observation, the organoid is expected to find wide applications such as screening methods for hair growth promoters, alopecia preventatives, alopecia mitigators, or alopecia treatments, and screening methods for cosmetic compositions for promoting hair growth, preventing alopecia, or mitigating alopecia. La présente invention concerne une technologie de construction et de culture d'organoïdes de follicules pileux pour des procédés de criblage de médicaments capillaires et la production de matériaux de transplantation capillaire. Plus particulièrement, la présente invention a permis d'isoler des follicules pileux à partir d'organoïdes cutanés issus de cellules souches pluripotentes induites humaines et de les cultiver sur des inserts de culture enduits de collagène et de Matrigel afin d'observer la croissance et la dégénérescence des follicules pileux. La transplantation des follicules pileux cultivés dans la peau a entraîné la croissance de nouveaux cheveux, ce qui confirme l'utilisation potentielle des organoïdes de follicules pileux comme matériel de transplantation capillaire. Un modèle imitant la perte de cheveux a été généré à partir de l'observation de réductions de la longueur des follicules pileux, de dommages aux agrégats de cellules de la papille pileuse, d'une diminution de l'expression de WNT3A ou de β-caténine, et d'une augmentation de l'expression de DKK-1 ou de TGF-β2 en fonction du traitement des organoïdes de follicules pileux avec des facteurs induisant l'alopécie. Grâce à cette observation, l'organoïde devrait trouver de vastes applications telles que les procédés de criblage pour les promoteurs de la croissance des cheveux, les préventifs de l'alopécie, les atténuateurs de l'alopécie ou les traitements de l'alopécie, ainsi que les procédés de criblage pour les compositions cosmétiques destinées à promouvoir la croissance des cheveux, à prévenir l'alopécie ou à atténuer l'alopécie. 본 발명은 모발 약물 스크리닝 방법 및 모발 이식 소재 제작을 위한 모낭 오가노이드 제작 및 배양기술에 관한 것으로, 보다 구체적으로, 인간 유도만능줄기세포주를 이용한 기존의 피부 오가노이드에서 모낭을 모항기에 분리하고 이를 콜라겐 및 마트리겔로 코팅된 배양 삽입물 위에서 배양하여 모낭의 성장과 퇴화를 확인하였으며, 배양된 모낭을 피부에 미식한 결과 새로운 모발이 성장함을 확인하여 모발 이식 소재로서 활용할 수 있으며, 모낭 오가노이드에 탈모 유발 요인 처리에 따라, 모낭의 길이 감소, 모유두세포 응집체 손상, WNT3A 또는 β-catenin의 발현 감소, DKK-1 또는 TGF-β2의 발현 증가를 확인하여 탈모 모사 모델을 생성한바, 이를 통해 발모 촉진제, 탈모 예방제, 탈모 완화제 또는 탈모 치료제 스크리닝 방법 또는 발모 촉진, 탈모 예방 또는 탈모 완화용 화장료 조성물 스크리닝 방법 등 다양하게 활용할 수 있을 것으로 기대된다.