INTRACELLULAR DELIVERY OF COMPOUNDS IN MULTIPLEXED CELL-BASED ASSAYS

• Main Authors: CASSADAY, Jason A, OTTEN, Stephanie, SQUADRONI, Brian, KASPER, Stephen H
• Format: Patent
• Language: English; French
• Published: 10.10.2024
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Systems and methods are provided for introducing exogenous material into cells in a fast, multiplexed manner in vitro using mechanoporation, e.g., microfluidic vortex shedding or cell squeezing. L'invention concerne des systèmes et des procédés pour introduire un matériau exogène dans des cellules d'une manière rapide et multiplexée in vitro à l'aide d'une mécanoporation, par exemple, d'un décollement de tourbillon microfluidique ou d'un pressage cellulaire.