METHODS AND SYSTEMS FOR POLYMER SYNTHESIS BY CONTACTING SYNTHESIS SURFACES WITH COMPARTMENTALIZED LIQUID REAGENTS

• Main Authors: FOSTER, Evan, SWAVOLA, Julia, HOFF, Christopher, TRAN, Tuan, BHAUMIK, Urmi, PALLUK, Sebastian, KHARITON, Margarita, KOSCINSKI, Joseph
• Format: Patent
• Language: English; French
• Published: 19.09.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

Disclosed herein are methods and devices for improved de novo cyclic enzymatic polymer synthesis using a protected monomer. Protected monomers include those with a protecting group that prevents addition of more than one monomer during a synthesis step, including the catalytic enzyme attached to the monomer. In specific embodiments, the polymer synthesis method is an enzymatic de novo polynucleotide synthesis method using protected nucleotides, such as nucleotides comprising a reversible terminator or nucleotides bound to a catalytic polymerase. L'invention concerne des procédés et des dispositifs pour une synthèse de polymère enzymatique cyclique de novo améliorée à l'aide d'un monomère protégé. Les monomères protégés comprennent ceux ayant un groupe protecteur qui empêche l'ajout de plus d'un monomère pendant une étape de synthèse, comprenant l'enzyme catalytique fixée au monomère. Dans des modes de réalisation spécifiques, le procédé de synthèse de polymère est un procédé de synthèse de polynucléotide de novo enzymatique utilisant des nucléotides protégés, tels que des nucléotides comprenant un ou plusieurs nucléotides réversibles liés à une polymérase catalytique.