METHOD OF DETECTING DISEASE-ASSOCIATED MARKERS IN A LIQUID BIOPSY SAMPLE USING EXTRACELLULAR VESICLES

• Main Author: IM, Hyungsoon
• Format: Patent
• Language: English; French
• Published: 31.10.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MEASURING; NANOTECHNOLOGY; PERFORMING OPERATIONS; PHYSICS; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; TESTING; TRANSPORTING

The present disclosure relates to devices (e.g., nano-plasmonic chips carrying arrays of nanowells) and methods for the detection of cancer (e.g., CCA). Specifically, extracellular vesicles (EVs) can be captured in the nanowells of the nano-plasmonic chips and are then subject to multi-channel imaging that detects the presence and/or level of biomarkers associated with cancers such as CCA. Accordingly, in one aspect, provided herein are methods of detecting extracellular vesicles (EVs) from cholangiocarcinoma (CCA) cells in a sample, the method comprising: obtaining a sample, e.g., from a subject; isolating EVs from the sample; capturing EVs isolated from the sample on a nano-plasmonic chip bearing an array of nanostructures coated with a layer of gold; and detecting the presence and/or levels of one or more of MUC 1, EGER, and EpCAM, wherein a higher expression level of one or more of MU Cl, EGFR, and EpCAM compared to a corresponding reference level indicates that the sample includes EVs from CCA cells. La présente divulgation concerne des dispositifs (par exemple, des puces nano-plasmoniques possédant des réseaux de nanopuits) et des procédés pour la détection d'un cancer (par exemple, CCA).