EXPRESSION OF HUMAN PAPILLOMA VIRUS HPV35 L1 PROTEIN, VIRUS-LIKE PARTICLES, AND PREPARATION METHOD THEREFOR

• Main Authors: CHEN, Xiao, YIN, Fei, WANG, Xuehong, ZHANG, Yao, GAO, Wenshuang, XUE, Junlian, CHEN, Dan, SHEN, Ercui, LIU, Yuying, ZHANG, Haijiang, LIU, Yongjiang, WU, Shuming
• Format: Patent
• Language: Chinese; English; French
• Published: 04.07.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

An expression of the human papilloma virus HPV35 L1 protein, virus-like particles, and a preparation method therefor. The preparation method comprises: truncating the amino acid sequence of the HPV35 L1 protein, carrying out codon optimization on a coding nucleotide sequence of the truncated protein to obtain an optimized coding nucleotide sequence, and lastly matching with a tag-free expression vector having a specific SD sequence to achieve tag-free expression and purification. By means of the described improvement, higher protein expression is obtained in prokaryotic expression systems such as an escherichia coli expression system, and VLPs having more consistent quality are obtained. L'invention concerne une expression de la protéine L1 du papillomavirus humain HPV35, des particules pseudo-virales et un procédé de préparation associé. Le procédé de préparation comprend : la troncature de la séquence d'acides aminés de la protéine L1 de HPV35, la réalisation d'une optimisation de codon sur une séquence nuc