IMPROVED ENZYMES AND METHODS FOR THE SYNTHESIS OF CANNABINOIDS

Disclosed herein are novel CBGA and CBGVA synthases and methods for improvement of their overall activities for the synthesis of CBGA and CBGVA from their respective precursors, olivetolic acid (OA) or divarinic acid (DVA) and GPP. Also disclosed are fusion proteins to enhance synthesis of CBGA and...

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Bibliographic Details
Main Authors KOMOR, Russell Scott, CAIAZZA, Nicky Christopher, KEUL, Nicholas Donald, WALKER, Caleb Marshall, KAMBOURAKIS, Spiros
Format Patent
LanguageEnglish
French
Published 08.08.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Disclosed herein are novel CBGA and CBGVA synthases and methods for improvement of their overall activities for the synthesis of CBGA and CBGVA from their respective precursors, olivetolic acid (OA) or divarinic acid (DVA) and GPP. Also disclosed are fusion proteins to enhance synthesis of CBGA and CBGVA. The methods described herein also increase the titer and the purity of CBGA and CBGVA made by a cell by 1) decreasing the formation of byproducts FCBGA and FCBGVA that are synthesized from the respective prenylation of OA and DVA with FPP, 2) increasing the intracellular availability of OA and DVA and 3) increasing the speed of CBGA and CBGVA formation, while reducing accumulation of intermediates. L'invention concerne de nouveaux CBGA et CBGVA synthases et des procédés pour l'amélioration de leurs activités globales pour la synthèse de CBGA et de CBGVA à partir de leurs précurseurs respectifs, l'acide olivetolique (OA) ou l'acide divarinique (DVA) et le GPP. L'invention concerne également des protéines de fusion pour améliorer la synthèse de CBGA et de CBGVA. Les procédés décrits ici augmentent également le titre et la pureté de CBGA et CBGVA fabriqués par une cellule en 1) diminuant la formation de sous-produits FCBGA et FCBGVA qui sont synthétisés à partir de la prénylation respective de OA et DVA avec FPP, 2) augmentant la disponibilité intracellulaire de OA et DVA et 3) augmentant la vitesse de formation de CBGA et CBGVA, tout en réduisant l'accumulation d'intermédiaires.
Bibliography:Application Number: WO2023US84953