EFFICIENT AND SCALABLE SUSPENSION IPSC CULTURE SYSTEM COMPLIANT WITH GOOD MANUFACTURING PRACTICES

• Main Authors: MARTIN-IBANEZ, Raquel, LEE, Hojae, SAREEN, Dhruv
• Format: Patent
• Language: English; French
• Published: 13.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Described herein are cGMP methods of iPSC adaptation and culturing in suspension culture. Also described are iPSC expansion methods in suspension culture. Various embodiments provide for a method of adapting adherent induced pluripotent stem cells (iPSCs) to suspension iPSCs, comprising: (i) seeding about 75,000-800,000 iPSCs per ml cGMP feeder-free iPSC maintenance medium; (ii) culturing the iPSCs for about 2.5-3.S days to allow the formation of iPSC spheres; (iii) culturing the iPSC spheres for about 3.5-4.S days, feeding the iPSC spheres about daily with the cGMP feeder-free iPSC maintenance medium; (iv) passaging the iPSC spheres; (v) culturing about 80,000-120,000 iPSC spheres for about 3.5-4.5 days in the cGMP feeder free iPSC maintenance medium; (vi) culturing the iPSC sphere for about 2.5-3.S days, feeding the culture about daily with the cGMP feeder-free iPSC maintenance medium; (vii) repeating steps (iv)-(vi) at.least once. L'invention concerne des procédés GMPc d'adaptation et de culture iPSC en culture en suspension. L'invention concerne également des procédés d'expansion iPSC dans une culture en suspension.