MUTANT RESPIRATORY SYNCYTIAL VIRUS PRE-FUSION F PROTEIN AND USE THEREOF

• Main Authors: HUANG, Lingling, SHI, Li, ZHANG, Jiancheng, CAO, Yufeng, CHEN, Xiaoyu, YI, Xiaonan, LIU, Lin, RUAN, Baoyang
• Format: Patent
• Language: Chinese; English; French
• Published: 30.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

Provided is a mutant respiratory syncytial virus pre-fusion F protein, having the following mutations compared with a wild-type respiratory syncytial virus pre-fusion F protein: (a) at least one amino acid residue in an F1 subunit and/or an F2 subunit is substituted by cysteine, and (b) amino acid residues at positions 104-144 of the wild-type respiratory syncytial virus pre-fusion F protein are partially or completely substituted by a linker peptide, at least two lengths of the amino acid residues of the linker peptide being comprised. Compared with similar products, the mutant respiratory syncytial virus pre-fusion F protein has excellent neutralizing antibody binding activity and thermal stability, and is suitable for being developed into one of components of respiratory syncytial virus vaccines. L'invention concerne une protéine F de pré-fusion du virus respiratoire syncytial mutant, ayant les mutations suivantes par comparaison avec une protéine F de pré-fusion du virus respiratoire syncytial de type sau