HIGH THROUGHPUT SOYBEAN ROOT DISEASE SCREEN

• Main Authors: MILACH, Sandra, FERREIRA, Adriana O, LOURENÇO TESSUTTI, Isabela Tristan, MORGANTE, Carolina Vianna, WOODWARD, John Bryan, BRUGIERE, Norbert, GUIMARÃES, Patricia Messenberg, BRASILEIRO, Ana Cristina Miranda, PEREIRA, Bruna Medeiros, ARRAES, Fabricio Barbosa Monteiro, GROSSI DE SA, Maria Fatima
• Format: Patent
• Language: English; French
• Published: 10.05.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Provided are methods for identifying polynucleotides that increase a plants resistance to a pathogen of interest (e.g., root knot nematode) using hairy-roots generated from leaves comprising providing a population of soybean leaves isolated from a soybean plant population comprising plants susceptible to a pathogen of interest, introducing into the population of soybean leaves a candidate resistance polynucleotide, stimulating hairy-root growth from the population of soybean leaves comprising the introduced candidate resistance polynucleotide, inoculating the hairy-roots with at least one pathogen of interest, and measuring the infection level of the at least one pathogen of interest on the inoculated hairy-roots. Additionally, provided are methods for screening plants for resistance to a pathogen of interest using hairy-roots generated from leaves. L'invention concerne des procédés d'identification de polynucléotides qui augmentent la résistance de plantes à un pathogène d'intérêt à l'aide de racines chevelues générées à partir de feuilles. De plus, l'invention concerne des procédés de criblage de plantes pour une résistance à un pathogène d'intérêt à l'aide de racines chevelues générées à partir de feuilles.