PERFUSABLE 3D TUBULE-ON-CHIP MODEL DERIVED FROM KIDNEY ORGANOIDS WITH IMPROVED DRUG UPTAKE

• Main Authors: ACEVES, Jeffrey, MORIZANE, Ryuji, LEWIS, Jennifer, WOLF, Kayla, VAN GAAL, Ronald, ROBINSON, Sanlin
• Format: Patent
• Language: English; French
• Published: 07.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Described herein are perfusable 3D tubule-on-chip models comprising at least one tubule consisting of one patent lumen circumscribed by organoid- derived cells, and a multifluidic platform comprising at least one individually addressable chip. The models may further include an unseeded tubule, where the seeded tubule and the unseeded tubule are co-localized on the chip, and wherein the tubule and the unseeded tubule are embedded within a gelatin-fibrin extracellular matrix (ECM). Also, described here are methods of producing the described perfusable 3D tubule-on-chip models, and uses of the same. La présente invention concerne des modèles de tubule 3D sur puce perfusables comprenant au moins un tubule constitué d'une lumière perméable circonscrite par des cellules dérivées d'organoïdes, et une plateforme multifluidique comprenant au moins une puce adressable individuellement. Les modèles peuvent en outre comprendre un tubule non ensemencé, le tubule ensemencé et le tubule non ensemencé étant co-localisés sur la puce, et le tubule et le tubule non ensemencé étant intégrés dans une matrice extracellulaire (MCE) de gélatine-fibrine. L'invention concerne également des méthodes de production des modèles de tubule 3D sur puce perfusables selon l'invention, et leurs utilisations.