METHOD FOR SELECTIVE DIFFERENTIATION INTO DORSAL-METENCEPHALIC TISSUE FROM EMBRYOID DERIVED FROM PLURIPOTENT STEM CELL

• Main Authors: YANG, Ji Hun, KWAK, Tae Hwan
• Format: Patent
• Language: English; French; Korean
• Published: 15.02.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for selective differentiation into dorsal-metencephalic tissue from embryoids derived from pluripotent stem cells, the method comprising the step of selective differentiation into dorsal-metencephalic tissues in which embryoids derived from pluripotent stem cells are cultured in the presence of a dorsal-metencephalic tissue-inducing medium containing a sonic hedgehog (SHh) signaling pathway inhibitor. La présente invention concerne un procédé de différenciation sélective en tissu dorso-métencéphalique à partir d'embryoïdes issus de cellules souches pluripotentes, le procédé comprenant une étape de différenciation sélective en tissu dorso-métencéphalique dans laquelle les embryoïdes issus de cellules souches pluripotentes sont cultivés en présence d'un milieu d'induction de tissu dorso-métencéphalique contenant un inhibiteur de la voie de signalisation Sonic Hedgehog (SHh). 본 발명은 SHh(sonic hedgehog) 신호전달계 억제제를 포함하는 등쪽 후뇌 조직 유도 배지의 존재 하에서, 전분화능 줄기세포로부터 유래된 배아체를 배양하는, 등쪽 후뇌 조직으로의 선택적 분화 단계를 포함하는, 전분화능 줄기세포로부터 유래된 배아체의 등쪽 후뇌 조직으로의 선택적 분화 방법에 관한 것이다.