NAKED EYE DETECTION METHOD FOR SARS-COV-2 RDRP MUTATIONS

• Main Authors: GUK, Kyeong Hye, YI, So Yeon, LIM, Eun Kyung, JUNG, Ju Yeon, KANG, Tae Joon, KIM, Hye Ran
• Format: Patent
• Language: English; French; Korean
• Published: 08.02.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a naked eye detection method for SARS-CoV-2 RdRp mutations. In the present invention, a colorimetric detection method using the CRISPR-Cas12a system was developed, allowing for easy diagnosis of SARS-CoV-2 RNA using the naked eye. Notably, the detection method according to the present invention is characterized by the ability to specifically and sensitively identify different mutations of SARS-CoV-2. La présente invention concerne un procédé de détection à l'œil nu des mutations RdRp du virus SARS-CoV-2. Dans la présente invention, un procédé de détection colorimétrique utilisant le système CRISPR-Cas12a a été mis au point, permettant de diagnostiquer facilement l'ARN du SRAS-CoV-2 à l'œil nu. Notamment, le procédé de détection selon la présente invention se caractérise par sa capacité à identifier spécifiquement et de manière sensible différentes mutations du SARS-CoV-2. 본 발명은 SARS-CoV-2 RdRp 변이 육안 검출법에 관한 것이다. 본 발명은 CRISPR-Cas12a 시스템을 이용한 비색 검출법을 개발하여 SARS-CoV-2의 RNA를 육안으로 쉽게 진단할 수 있다. 특히, 본 발명에 따른 검출법은 SARS-CoV-2의 변이 종류를 특이적으로 민감하게 식별할 수 있는 특징이 있다.