RECOMBINANT PROTEINS, RECOMBINANT DNA SEQUENCES, VECTORS, EUKARYOTIC AND PROKARYOTIC EXPRESSION SYSTEMS AND THEIR USE

• Main Authors: CECUDA-ADAMCZEWSKA, Violetta, DOMANSKI, Sylwester, ZAKRZEWSKA, Agnieszka, WSZOLA, Michal, KLAK, Marta, LUKASIEWICZ, Natalia, ROMANIKRUSCIELEWSKA, Agnieszka, FLORYS-JANKOWSKA, Katarzyna, SOKOLOWSKA, Iwona
• Format: Patent
• Language: English; French
• Published: 20.06.2024
• Subjects: ADDITIVE MANUFACTURING TECHNOLOGY; ADDITIVE MANUFACTURING, i.e. MANUFACTURING OFTHREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVEAGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING,STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING; ADHESIVES; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMICAL PAINT OR INK REMOVERS; CHEMISTRY; COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS; CORRECTING FLUIDS; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; DYES; FILLING PASTES; HUMAN NECESSITIES; HYGIENE; INKS; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; NATURAL RESINS; ORGANIC CHEMISTRY; PAINTS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; PEPTIDES; PERFORMING OPERATIONS; POLISHES; TRANSPORTING; USE OF MATERIALS THEREFOR; WOODSTAINS

The subject of the invention is a recombinant hybrid protein RElSmR composed sequentially of 3 resilin domains, 1 K+ domain, 1 MMP domain, 3 elastin domains, 1 K+ domain, 4 elastin domains, 1 K+ domain, 4 elastin domains, 1 K+ domain, 3 elastin domains, 1 MMP domain, 7 RGD domains. The subject of the invention also includes a recombinant hybrid protein EJ17zipR, composed sequentially of 1 ZIP domain, 56 elastin domains, 9 silk fibroin domains, 7 RGD domains, 56 elastin domains, 10 silk fibroin domains, 7 RGD domains. The subject of the invention also includes recombinant DNA sequences encoding recombinant hybrid proteins of the invention, vectors comprising said recombinant DNA sequences of the invention, and eukaryotic or prokaryotic expression systems transformed with said recombinant DNA sequences of the invention. Finally, the subject of the invention includes uses of the recombinant hybrid proteins of the invention, and the use of the recombinant DNA sequences, vectors and eukaryotic or prokaryotic expression systems of the invention. L'invention a pour objet une protéine hybride recombinée RElSmR composée séquentiellement de : 3 domaines résiline, 1 domaine K+, 1 domaine MMP, 3 domaines élastine, 1 domaine K+, 4 domaines élastine, 1 domaine K+, 4 domaines élastine, 1 domaine K+, 3 domaines élastine, 1 domaine MMP, 7 domaines RGD. L'invention concerne également une protéine hybride recombinée EJ17zipR, composée séquentiellement de : 1 domaine ZIP, 56 domaines élastine, 9 domaines fibroïne de soie, 7 domaines RGD, 56 domaines élastine, 10 domaines fibroïne de soie, 7 domaines RGD. L'invention concerne également des séquences d'ADN recombinées codant pour des protéines hybrides recombinées de l'invention, des vecteurs comprenant lesdites séquences d'ADN recombinées de l'invention, et des systèmes d'expression eucaryotes ou procaryotes transformés avec lesdites séquences d'ADN recombinées de l'invention. Enfin, l'invention concerne des utilisations des protéines hybrides recombinées de l'invention, et l'utilisation des séquences d'ADN recombinées, des vecteurs et des systèmes d'expression eucaryotes ou procaryotes de l'invention.