COMPOSITIONS, KITS, AND METHODS FOR DETECTING NUCLEIC ACIDS USING INTRA-CHANNEL MULTIPLEXING

• Main Authors: EVANS, Brian, LEONG, Harrison, ZAHEDI, Mohammad Mehdi, SHANNON, Mark, MULLAH, Khairuzzaman Bashar, GJERSTAD, Carmen
• Format: Patent
• Language: English; French
• Published: 04.01.2024
• Subjects: ADHESIVES; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DYES; ENZYMOLOGY; LAKES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; MORDANTS; MUTATION OR GENETIC ENGINEERING; NATURAL RESINS; ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; PAINTS; POLISHES; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Disclosed are compositions, kits, and methods that enable intra-channel multiplexing by enabling determination of separate detectable signals, each associated with a different assay target, within the same detection channel. The multiple detectable signals can be separately resolved and independently analyzed to enable detection and/or quantification of each respective target. Enabling multiple targets to be assayed within the same detection channel increases the plexy of multiplex assays without relying on additional dyes and concomitant issues of increased spectral overlap. Sont divulgués des compositions, des kits et des méthodes qui permettent un multiplexage intra-canal en permettant la détermination de signaux détectables séparés, chque signal associé à une cible de dosage différente, dans le même canal de détection. Les multiples signaux détectables peuvent être résolus séparément et analysés indépendamment pour permettre la détection et/ou la quantification de chaque cible respective. L'activation de multiples cibles dans le même canal de détection augmente la variété de dosages multiplex sans dépendre de colorants supplémentaires et de problèmes concomitants de chevauchement spectral accru.