METHODS FOR MAKING HIGH INTENSITY SWEETENERS

• Main Authors: PATRON, Andrew, NORIEGA, Chris Edano, MANAM, Rama Rao
• Format: Patent
• Language: English; French
• Published: 02.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

Provided herein include methods of making mogroside compounds, e.g., siamenoside I 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., siamenoside I). Also provided herein are novel cucurbitadienol synthases and the use thereof. La présente invention concerne des procédés de fabrication de composés mogrosides, par exemple du siaménoside I 1, des compositions (par exemple des cellules hôtes) pour fabriquer les composés mogrosides et les composés mogrosides fabriqués par les procédés de l'invention, ainsi que des compositions (par exemple des lysats cellulaires) et des cellules recombinées comprenant les composés mogrosides (par exemple du siaménoside I 1). L'invention concerne également de nouvelles cucurbitadiénol synthases et leur utilisation.