MONKEYPOX VIRUS-SPECIFIC DETECTION TARGET, AND OLIGONUCLEOTIDE AND KIT THEREOF

• Main Authors: ZHANG, Taisong, LIU, Xuefeng, JIAO, Mingchao, CHEN, Feifei
• Format: Patent
• Language: Chinese; English; French
• Published: 14.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a monkeypox virus-specific detection target, and an oligonucleotide, a kit and a method for detecting a monkeypox virus. The method comprises designing a specific primer and probe for an F3L gene region in a monkeypox virus genome, and utilizing fluorescent PCR technology to detect monkeypox virus nucleic acid after rapid extraction of a nucleic acid from a sample. The operation is simple, and a monkeypox virus target gene is detected by using a TaqMan probe, so that a vaccinia virus with very high sequence similarity can be specifically distinguished. False detection and detection failure can be avoided. La présente invention concerne une cible de détection spécifique du virus de la variole du singe, ainsi qu'un oligonucléotide, un kit et un procédé de détection d'un virus de la variole du singe. Le procédé consiste à concevoir une amorce et une sonde spécifiques pour une région du gène F3L dans le génome du virus de la variole du singe, et à utiliser la technologie PCR fluor