METHOD FOR CONSTRUCTING GRAM-NEGATIVE BACTERIAL OUTER MEMBRANE-DERIVED NANOVESICLES AND USE THEREOF

• Main Authors: LEE, Jie Oh, BAE, Seo Yoon, GHO, Yong Song, SHIN, Ha Young, SONG, Sung Hyun, LEE, Jae Wook, ROH, Tae Young, JEONG, Hye Jin, LEE, Tae Ryong, YOO, Mi Joung, LEE, Chang Jin
• Format: Patent
• Language: English; French; Korean
• Published: 30.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for constructing Gram-negative bacterial outer membrane-derived nanovesicles and a use thereof. Also, the present invention provides a method for treating or diagnosing cancer using Gram-negative bacterial outer membrane-derived nanovesicles. In addition, the present invention provides a method for delivering therapeutic or diagnostic materials to target cells or tissues by loading same into Gram-negative bacterial outer membrane-derived nanovesicles. Furthermore, the present invention provides a method for the prevention and treatment of various diseases including cancer and bacterial/viral infections by co-administering Gram-negative bacterial outer membrane-derived nanovesicles and antigens or by administering Gram-negative bacterial outer membrane-derived nanovesicles having antigens expressed thereon. La présente invention concerne un procédé de construction de nanovésicules issues de la membrane externe de bactéries gram-négatives et son utilisation. La présente invention concerne également un procédé de traitement ou de diagnostic du cancer utilisant des nanovésicules issues de la membrane externe de bactéries gram-négatives. En outre, la présente invention concerne un procédé permettant de délivrer des substances thérapeutiques ou diagnostiques à des cellules ou tissus cibles en les chargeant dans des nanovésicules issues de la membrane externe de bactéries gram-négatives. En outre, la présente invention concerne un procédé de prévention et de traitement de diverses maladies, y compris le cancer et les infections bactériennes/virales, par l'administration conjointe de nanovésicules issues de la membrane externe de bactéries gram-négatives et d'antigènes ou par l'administration de nanovésicules issues de la membrane externe de bactéries gram-négatives présentant des antigènes exprimés sur ces dernières. 본 발명은 그람 음성 박테리아 외막 유래 나노소포체(Gram-negative bacterial outer membrane-derived nanovesicles)의 제조 방법 및 이의 용도에 관한 것이다. 또한 본 발명은 그람 음성 박테리아 외막 유래 나노소포체를 이용하여 암을 치료 또는 진단하는 방법을 제공한다. 이에 더하여, 본 발명은 그람 음성 박테리아 외막 유래 나노소포체에 질병 치료용 또는 진단용 물질을 로딩하여 표적 세포나 조직에 전달하는 방법을 제공한다. 또한 그람 음성 박테리아 외막 유래 나노소포체를 항원과 병용 투여하거나, 항원이 발현된 그람 음성 박테리아 외막 유래 나노소포체를 투여하여 암을 비롯한 다양한 질병 및 박테리아/바이러스 감염 등을 예방 및 치료하는 방법을 제공한다.