GENE MANIPULATION BASED ON NANOPARTICLE-CRISPR COMPLEX AND FABRICATION METHOD THEREFOR

• Main Authors: JOO, Minju, LEE, Kangseok, YEOM, Jihyun, HYEON, Hana, JEONG, Jaecheol, KIM, Chayoung, RYU, Minkyung, BAE, Jeehyeon, SIM, Sehoon, PARK, Sungchul, JEONG, Yujeong
• Format: Patent
• Language: English; French; Korean
• Published: 23.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NANOTECHNOLOGY; PERFORMING OPERATIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; SPIRITS; TRANSPORTING; VINEGAR; WINE

The present invention pertains to a nanoparticle delivery vehicle designed to bind Cas protein and guide RNA, a nano-complex containing the bound Cas protein and guide RNA, and a gene editing method using same. The use of the nano-complex of the present invention allows for stable and effective delivery of the guide RNA and the Cas protein at the same time into the target cells. La présente invention concerne un véhicule d'administration de nanoparticules conçu pour se lier à la protéine Cas et à l'ARN guide, un nano-complexe contenant la protéine Cas et l'ARN guide liés, et un procédé d'édition de gène l'utilisant. L'utilisation du nano-complexe de la présente invention permet une administration stable et efficace de l'ARN guide et de la protéine Cas simultanément dans les cellules cibles. 본 발명은 Cas 단백질 및 가이드 RNA가 결합하도록 제작된 나노 입자 전달체 및 이에 결합된 Cas 단백질 및 가이드 RNA를 포함하는 나노 복합체 및 이를 이용한 유전자 편집 방법에 대한 것이다. 본 발명의 상기 나노 복합체를 이용할 경우, 목적 세포 내로 가이드 RNA 및 Cas 단백질을 동시에 안정적이고 효과적으로 전달할 수 있다.