MICROFLUIDIC CHIP AND METHODS FOR CULTURING SINGLE CELL AND SCREENING AND EXPORTING CELL POPULATION

A microfluidic chip and methods for culturing a single cell and screening and exporting cell population. The chip (1) comprises a substrate layer, a flow channel layer, an inlet flow channel (2), an outlet flow channel (3), a plurality of common flow channels (4), and a plurality of functional units...

Full description

Saved in:
Bibliographic Details
Main Authors WANG, Kun, GUAN, Yimin, YANG, Miaomiao
Format Patent
LanguageChinese
English
French
Published 05.10.2023
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:A microfluidic chip and methods for culturing a single cell and screening and exporting cell population. The chip (1) comprises a substrate layer, a flow channel layer, an inlet flow channel (2), an outlet flow channel (3), a plurality of common flow channels (4), and a plurality of functional units (5), wherein the flow channel layer is located below the substrate layer, and the materials of the flow channel layer comprise at least one of silicon dioxide, spin-on glass, non-photosensitive epoxy resin, and non-photosensitive polyimide; two ends of the common flow channel (4) are connected to the inlet flow channel (2) and the outlet flow channel (3), respectively; the functional unit (5) comprises a single cell introduction port (501), a cell culturing-screening chamber (502), a cell exporting chamber (503), a cell exporting port (504), and a driving element (505), the driving element (505) being used for providing power for liquid so as to introduce a single cell (6) entering the common flow channel (4) into
Bibliography:Application Number: WO2022CN91720