CELL CULTURE METHODS FOR ANTIBODY PRODUCTION

• Main Authors: CROWLEY, John, LAFOND, Michelle, OSHODI, Shadia Abike, TANG, Xiaolin, CORBETT, Daniel, REILLY, James, VARTAK, Ankit, CHIBOROSKI, Mark, TUSTIAN, Andrew, STARLING, Alessandra, GOH, Hai-yuan, NICHOLL, Liam, HOURIHAN, John, WITMER, Ashley, MELLORS, Philip, STAIRS, Robert, MATTILA, John, JOHNSON, Amy S, BAK, Hanne, LAWRENCE, Shawn M, CASEY, Meghan, CALLINAN, Laura, CONLON, Aishling
• Format: Patent
• Language: English; French
• Published: 07.09.2023
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure. La présente invention concerne des procédés de fabrication de produits d'anticorps à titre élevé. En particulier, l'invention concerne, en partie, un milieu de culture de cellules animales sans sérum amélioré, qui peut être utilisé pour la production d'une protéine d'intérêt. De plus, la présente invention concerne en outre des procédures chromatographiques utilisées pour isoler avec succès le produit d'anticorps selon la présente invention.