C-TERMINAL PEPTIDE EXTENSIONS WITH INCREASED ACTIVITY

The disclosure provides Moloney murme leukemia virus (MMLV ) reverse transcriptase (RTase) mutants with C-terminal peptide and/or N-terminal peptide extensions that improve the performance of the MMLV RTase mutants. The disclosure also provides suitable amino acid positions in MMLV RTases for mutage...

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Bibliographic Details
Main Authors REEB, Tanner, VAKULSKAS, Christopher, BEAUDOIN, Sarah
Format Patent
LanguageEnglish
French
Published 28.09.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The disclosure provides Moloney murme leukemia virus (MMLV ) reverse transcriptase (RTase) mutants with C-terminal peptide and/or N-terminal peptide extensions that improve the performance of the MMLV RTase mutants. The disclosure also provides suitable amino acid positions in MMLV RTases for mutagenesis and methods and kits for using MMLV RTase mutants to synthesize cDNA from RNA templates. L'invention concerne des mutants de la transcriptase inverse (RTase) du virus de la leucémie murine de Moloney (MoMuLV) avec des extensions de peptides C-terminaux et/ou de peptides N-terminaux qui améliorent les performances des mutants de la MoMuLV RTase. L'invention concerne également des positions d'acides aminés appropriées dans des MoMuLV RTases pour la mutagenèse ainsi que des procédés et des kits d'utilisation de mutants de MoMuLV RTase pour synthétiser l'ADNc à partir de modèles d'ARN.
Bibliography:Application Number: WO2023US63407