CO-CULTURE SYSTEMS AND METHODS

• Main Authors: KO, Hui Ling, REN, Ee Chee
• Format: Patent
• Language: English; French
• Published: 23.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

This invention relates to a co-culture system comprising a first population of cells and a second population of HBV producing cells separated from the first population of cells, wherein the first population of cells is capable of being continuously infected by the HBV continuously produced by the second population of HBV producing cells. The co-culture system is used for screening for at least one agent against HBV infection and identifying at least one agent as a HBV cccDNA-directed agent. In addition, methods for preventing or inhibiting cccDNA formation of HBV or HBV infection in a subject using compound selected from the group consisting of Bilastine, Pitolisant and Nizatidine, or a derivative thereof, are also provided. L'invention concerne de manière générale le domaine de la virologie. En particulier, l'invention porte sur un système de co-culture et une méthode de criblage d'au moins un agent contre une infection par le VHB à l'aide du système de co-culture.