OFF-THE-SHELF HUMAN UMBILICAL CORD DERIVED MESENCHYMAL STEM CELL, PREPARATION METHOD AND APPLICATION THEREOF

• Main Authors: WANG, Lei, ZHANG, Yu, QI, Qi, ZHOU, Wangyi, ZHANG, Yun, HOU, Ruizhen, WU, Wenjie
• Format: Patent
• Language: Chinese; English; French
• Published: 06.07.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides an off-the-shelf human umbilical cord derived mesenchymal stem cell, a preparation method thereof, and an application. The preparation method comprises the following steps: culturing an isolated umbilical cord tissue block to obtain a P0-generation cell; passaging the P0-generation cell twice to obtain a P2-generation cell and cryopreserving same; resuscitating the cryopreserved P2-generation cell, passaging twice to obtain a P4-generation cell and cryopreserving same; resuscitating the cryopreserved P4-generation cell, passaging once to obtain a P5-generation cell, and after sequential digestion, washing and resuspension of same, obtaining a human umbilical cord derived mesenchymal stem cell. Further, adopting a cryopreservation storage method, a human umbilical cord derived mesenchymal stem cell cryopreserved preparation is developed. The cryopreserved preparation is convenient to store, and after resuscitation, intravenous drug administration can be directly carried out, with no liqu