ANTI-B7-H4 ANTIBODY, AND PREPARATION METHOD THEREFOR AND USE THEREOF

• Main Authors: DING, Yi, WU, Xiaodong, JIA, Gezi, WANG, Yongqiang, HE, Yun, RONG, Yiping, LI, He, CHEN, Fei, ZHAO, Chuchu, NIU, Lei
• Format: Patent
• Language: Chinese; English; French
• Published: 23.02.2023
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

Provided is an anti-B7-H4 antibody containing a VH, wherein the VH contains the following CDRs or a mutation thereof: CDR1 as shown in the amino acid sequence of SEQ ID NO: 4 or 5, CDR2 as shown in the amino acid sequence of SEQ ID NO: 15, and CDR3 as shown in the amino acid sequence of SEQ ID NO: 24 or 25, and wherein the mutation is an insertion, deletion or substitution of 3, 2 or 1 amino acid(s) in the amino acid sequences of the CDRs. The antibody has the activity of binding to human B7-H4 and cynomolgus monkey B7-H4. The antibody still retains the activity of binding to human B7-H4 and cynomolgus monkey B7-H4 when prepared into a B7-H4×CD3 bispecific antibody, and has a strong killing effect on tumor cells. The antibody induces a very low expression of nonspecific cytokines such as IL-6 and IFN-γ, and exhibits a strong in vivo anti-tumor activity. L'invention concerne un anticorps anti-B7-H4 contenant une VH, la VH contenant les CDR suivantes ou une mutation de celles-ci : CDR1 telle que représentée dan