CELL-SURFACE ENGINEERING COMPOSITIONS AND METHODS FOR THE PREVENTION OF IMMUNE REJECTION

• Main Authors: SIREN, Erika, CHOY, Jonathan, DU, Caigan, LUO, Haiming, WITHERS, Stephen, KIZHAKKEDATHU, Jayachandran, ENNS, Winnie
• Format: Patent
• Language: English; French
• Published: 29.12.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TRAPPING; VINEGAR; WINE

Provided herein are compounds including a linear polyglycerol, a peptide tag, with either a linker-sugar-sialic acid moiety or a sulfate group, a preservation solutions including the compounds, methods for using the compounds or the preservation solutions to cell surface engineering (CSE) of a cell, a tissue, an organ for transplant, and methods for making the compounds. In particular, the CSE may recapitulate or rebuild glycocalyx on the luminal endothelial surface of an organ to limit immune rejection of the organ after transplant. L'invention concerne des composés comprenant un polyglycérol linéaire, une étiquette peptidique, ayant soit une fraction de liaison sucre-acide sialique, soit un groupe sulfate, des solution de conservation comprenant les composés, des procédés d'utilisation des composés ou des solutions de conservation dans la modification de surface cellulaire (CSE) d'une cellule, d'un tissu, d'un organe de greffe, et des procédés de fabrication des composés. En particulier, la CSE peut reproduire ou reconstruire le glycocalyx sur la surface endothéliale luminale d'un organe pour limiter le rejet immunitaire de l'organe après transplantation.