IMPROVED METHODS FOR PRODUCING RECOMBINANT PROTEINS

Recombinant proteins comprising a non-canonical amino acid with high yield and high fidelity are made by expressing the protein in an engineered Vibrio natriegens strain containing an orthogonal translation system comprising an orthogonal aminoacyl tRNA synthetase that charges the non-canonical amin...

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Bibliographic Details
Main Authors SANTIAGO GONZÁLEZ, Sebasthian, SHRADER, Alanna Schepartz
Format Patent
LanguageEnglish
French
Published 22.12.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Recombinant proteins comprising a non-canonical amino acid with high yield and high fidelity are made by expressing the protein in an engineered Vibrio natriegens strain containing an orthogonal translation system comprising an orthogonal aminoacyl tRNA synthetase that charges the non-canonical amino acid onto the orthogonal cognate tRNA. Protéines recombinées comprenant un acide aminé non canonique avec un rendement et une fidélité élevés produites par l'expression de la protéine dans une souche Vibrio natriegens modifiée contenant un système de traduction orthogonal comprenant une aminoacyl ARNt synthétase orthogonale chargeant l'acide aminé non canonique sur l'ARNt apparenté orthogonal.
Bibliography:Application Number: WO2022US33811