SARS-COV-2 IMMUNOASSAY METHOD AND IMMUNOASSAY KIT

• Main Authors: OCHIAI Yasushi, HIROTA Jiro, OKUYAMA Shinya, WATANABE Keisuke, ASAI Tomohide, UNO Satoru, ITO Shizuka
• Format: Patent
• Language: English; French; Japanese
• Published: 22.12.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention provides an immunoassay method for immunologically assaying SARS-CoV-2 in a biological sample, said method comprising using two types of monoclonal antibodies or antibody fragments thereof binding to a peptide fragment consisting of not more than 30 consecutive amino acids in the nucleocapsid protein of SARS-CoV-2, wherein the two types of monoclonal antibodies or antibody fragments thereof recognize different epitopes respectively. La présente invention concerne un procédé de dosage immunologique pour le dosage immunologique du SARS-CoV-2 dans un échantillon biologique, ledit procédé comprenant l'utilisation de deux types d'anticorps monoclonaux ou de fragments d'anticorps de ceux-ci se liant à un fragment peptidique constitué d'au plus 30 acides aminés consécutifs dans la protéine de nucléocapside du SARS-CoV-2, les deux types d'anticorps monoclonaux ou de fragments d'anticorps de ceux-ci reconnaissant respectivement différents épitopes. 本発明により、生体試料中のＳＡＲＳ－ＣｏＶ－２の免疫測定方法であって、ＳＡＲＳ－ＣｏＶ－２のヌクレオカプシドタンパク質中の連続する３０以下のアミノ酸から成るペプチド断片に結合するモノクローナル抗体又はその抗体断片を２種類用い、前記２種類のモノクローナル抗体又はその抗体断片が、それぞれ、異なるエピトープを認識する、免疫測定方法が提供される。