RNA-MODIFYING CHIMERIC PROTEIN AND APPLICATION THEREOF

• Main Authors: GAO, Haixia, DONG, Yingying, SHAO, Meiqi, QIAN, Qijun, LIU, Tao, SUN, Juanjuan, ZHANG, Pingjing, JIANG, Ting, QIU, Kaijun
• Format: Patent
• Language: Chinese; English; French
• Published: 13.10.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention provides a chimeric protein subunit, comprising the D12 subunits of (a)RNA capping enzymes connected to each other or functional fragments thereof, or a variant having at least 90% sequence identity thereto and having D12 subunit activity; and 2'-O-methytranseferase having a (b)RNA cap structure or a functional fragment thereof, or a variant having at least 90% sequence identity thereto and having the methytransefer activity of an RNA cap structure. The present invention further provides a chimeric protein containing the chimeric protein subunit and the D1 subunit of an RNA capping enzyme or the functional fragment thereof. La présente invention concerne les éléments suivants : une sous-unité protéique chimérique, comprenant les sous-unités D12 (a) d'enzymes de coiffage d'ARN reliées l'une à l'autre ou des fragments fonctionnels de celles-ci, ou un variant ayant une identité de séquence d'au moins 90 % avec celles-ci et ayant une activité de sous-unité D12 ; et une 2'-O-méthytransférase