ENZYME VARIANTS

• Main Authors: FLEISHMAN, Sarel-Jacob, KHERSONSKY, Olga, RACKHAM, Oliver, FILIPOVSKA, Aleksandra, VOS, Pascal
• Format: Patent
• Language: English; French
• Published: 16.06.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided herein are Cas9 proteins comprising SEQ ID NO:1 or a sequence at least 80% identical thereto, wherein: the amino acid residues at positions 765 to 780 are replaced by the amino acid sequence of SEQ ID NO:5 or SEQ ID NO:6; the amino acid residues at positions 838 to 853 are replaced by the a...