COMPOSITION FOR DETECTING TARGET NUCLEIC ACID AND METHOD FOR DETECTING TARGET NUCLEIC ACID USING SAME

• Main Authors: LIM, Jae Woo, LIM, Eun Kyung, JUNG, Ju Yeon, JANG, Soo Jin, KANG, Tae Joon, SEO, Seung Beom, KANG, Byung Hoon
• Format: Patent
• Language: English; French; Korean
• Published: 19.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The present invention relates to a technology for analyzing and detecting/diagnosing a target nucleic acid. When the detection system according to the present invention is used, effective real-time detection or diagnosis efficiency can be obtained while problems such as noise are minimized. In particular, since, by using a house-keeping gene according to a method of use, the expression difference of the target nucleic acid can be corrected, and direct real-time target nucleic acid detection is possible, it can be effectively used for detecting various nucleic acids and diagnosing various diseases thereby. La présente invention concerne une technologie d'analyse et de détection/diagnostic d'un acide nucléique cible. Lorsque le système de détection selon la présente invention est utilisé, une efficacité de détection ou de diagnostic en temps réel peut être obtenue tandis que les problèmes tels que le bruit sont minimisés. En particulier, étant donné que, en utilisant un gène domestique selon un procédé d'utilisation, la différence d'expression de l'acide nucléique cible peut être corrigée, et que la détection directe en temps réel de l'acide nucléique cible est possible, il peut être utilisé efficacement pour détecter divers acides nucléiques et diagnostiquer ainsi diverses maladies. 본 발명은 표적 핵산을 분석, 검출/진단하는 기술에 관한 것이다. 본 발명에 따른 검출 시스템을 이용하는 경우 노이즈 등의 문제를 최소화하면서 효과적인 실시간 검출 또는 진단 효율을 나타낼 수 있다. 특히, 이용 방법에 따라 항존유전자를 사용함으로써 표적 핵산의 발현 차이를 보정할 수 있고, 직접적인 실시간 표적 핵산 검출이 가능하므로, 다양한 핵산 검출 및 이에 따른 각종 질병 진단 등에 효과적으로 사용될 수 있다.