VIRAL VECTOR COMPRISING SARS-COV-2 ANTIGEN, AND USE THEREOF

• Main Authors: JUNG, Seo-yeon, SEO, Ki-weon, LEE, Kun Se
• Format: Patent
• Language: English; French; Korean
• Published: 05.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention relates to: a nucleic acid construct in which a heterogenous polynucleotide encoding at least one structural protein of SARS-CoV-2 or a variant thereof is inserted into a cDNA molecule encoding a full-length measles virus (MV) antigenomic (+) RNA strand, the at least one structural protein being selected from the group consisting of spike (S), nucleocapsid (N), and membrane (M) proteins; a viral vector comprising the construct; and a composition, preferably, a vaccine, for preventing SARS-CoV-2 infection, comprising the construct. A vaccine of the present invention is safe and has the excellent effect of inducing cell-mediated immune responses. La présente invention concerne les éléments suivants : une construction d'acide nucléique dans laquelle un polynucléotide hétérogène codant pour au moins une protéine structurale du SARS-CoV-2 ou un variant de celui-ci est inséré dans une molécule d'ADNc codant pour un brin d'ARN antigénomique (+) complet du virus de la rougeole (MV), la au moins une protéine structurale étant choisie dans le groupe constitué par les protéines de spicule (S), de nucléocapside (N) et de membrane (M) ; un vecteur viral comprenant la construction ; et une composition, de préférence, un vaccin, pour prévenir l'infection par le SRAS-CoV-2, comprenant la construction. Un vaccin issu de la présente invention est sûr et a un excellent effet d'induction de réponses immunitaires à médiation cellulaire. 본 발명은 홍역 바이러스(measles virus: MV)의 전장 길이 항게놈성 (+) RNA 가닥을 암호화하는 cDNA 분자에, 사스-코로나 바이러스-2의 적어도 하나의 구조 단백질 또는 이의 변이체를 암호화하는 이종의 폴리뉴클레오티드가 삽입된 핵산 작제물로, 상기 적어도 하나의 구조 단백질은 스파이크 (S) 단백질, 뉴클레오캡시드 (N) 단백질, 및 멤브레인 (M) 단백질로 이루어진 군에서 선택된 어느 하나의 단백질인 핵산 작제물, 이를 포함하는 바이럴 벡터 및 이를 포함하는 사스-코로나 바이러스-2 감염 예방용 조성물, 바람직하게 백신에 관한 것이다. 본 발명의 백신은 안전하면서, 세포성 면역 반응 유도 효과가 우수하다.