METHOD FOR ISOLATING DISEASE-SPECIFIC EXOSOME

• Main Authors: KIM, Ryun Hyung, JEONG, Hye In, MUN, Byeong Geol, HAAM, Seung Joo, KANG, Byung Hoon
• Format: Patent
• Language: English; French; Korean
• Published: 05.05.2022
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The present invention relates to a method whereby multiple exosomes overexpressing a disease-specific marker are isolated at the same time from the total population of exosomes. Compared to existing exosome isolation methods, in which exosomes are isolated from the total population of exosomes and then disease-related exosomes are isolated from the exosomes isolated from the total population, the method is simple and has the advantage of being able to concentrate and isolate only the disease-related exosomes, and the isolated disease-related exosome can be used for diagnosing and predicting the prognosis of diseases. La présente invention concerne un procédé permettant d'isoler simultanément plusieurs exosomes surexprimant un marqueur spécifique à une maladie à partir de la population totale d'exosomes. Par comparaison avec les procédés existants d'isolement des exosomes, consistant à isoler les exosomes de la population totale d'exosomes, puis à isoler les exosomes liés à la maladie à partir des exosomes isolés de la population totale, le procédé est simple et présente l'avantage de pouvoir concentrer et isoler uniquement les exosomes liés à la maladie, et l'exosome isolé lié à la maladie peut être utilisé pour diagnostiquer et prédire le pronostic des maladies. 본 발명은 질병 특이적 마커를 과발현하는 엑소좀을 전체 엑소좀과 한번에 다중 분리하는 방법에 관한 것으로, 전체 엑소좀을 분리한 후 질병 관련 엑소좀을 다시 분리하는 기존의 엑소좀 분리 방법과 비교하여 공정이 간단하고 질병 관련 엑소좀만 농축 분리할 수 있는 장점이 있으며, 분리된 질병 특이적 엑소좀은 질병의 진단 및 예후 예측에 활용될 수 있다.