SUSCEPTIBILITY DETECTION KIT FOR SUDDEN CARDIAC DEATH BASED ON INSERTION-DELETION POLYMORPHIC SITE OF HSPA1B GENE

• Main Authors: ZHANG, Qing, GAO, Yuzhen, HE, Yan, YU, Huan
• Format: Patent
• Language: Chinese; English; French
• Published: 21.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Disclosed is a susceptibility detection kit for sudden cardiac death (SCD) based on an insertion-deletion polymorphic site of the HSPA1B gene. A specific primer pair contained in the kit is designed for an rs3036297 insertion-deletion site on the HSPA1B gene. DNA fragments containing the site can be specifically amplified and different genotypes are identified by means of detecting the mobility of fragments with different lengths during capillary electrophoresis. In view of a case-control study, it is found that a subject who carries a deletion allele at the rs3036297 insertion-deletion site on the HSPA1B gene of tested DNA can be considered as susceptible to SCD. The technique can play a role in predicting the susceptibility of an individual to SCD by means of detecting the genotype of the rs3036297 insertion-deletion site on the HSPA1B gene of the individual. It is confirmed that a five-base (AAGTT) insertion-deletion polymorphism (rs3036297) in the 3'UTR of the HSPA1B gene is significantly correlated with